ABSTRACT
One of the problems of using high concentrations of cryoprotectants for sperm vitrification is the cytotoxic effect that affects sperm recovery after warming. Therefore, in this study, we determined the recovery rate after vitrification with and without cryoprotectants. Ejaculates with progressive motility and viability above 50% were used as samples. The samples were divided into two groups: (1) samples were mixed with a basic solution (2) samples were mixed with a vitrification solution. Sperm were vitrified by direct plunging into LN2. Sperm motility and viability were observed to evaluate the quality of sperm before and after vitrification. Overall, the sperm samples vitrified with cryoprotectants had a significantly higher proportion of sperm motility (56%) and viability (58.15%) compared with those vitrified without cryoprotectants (35% and 48%, respectively, p < 0.05). However, vitrification of human sperm without cryoprotectants could be recommended for routine assisted reproductive technology.
Keywords: sperm vitrification, cryoprotectants, sperm motility, sperm viability
