ABSTRACT
Recently, fungal systems have gained immense importance as model systems for genetic research due to its amenability to genetic manipulation. For genetic manipulation, the establishment of transformation system is the prerequisite. Fungal transformations have come a long way since last few decades and has enabled researchers to develop several model fungal systems. Researchers have developed different transformation techniques and gene functional analytical techniques for fungal systems. Both physical and biological methods of transformations techniques have been developed in fungus. This chapter discusses about the recent advancements in different transformation techniques including some major physical methods like protoplast transformation, lithium acetate method, bolistic method, electroporation and also the most popular biological method, the Agrobacterium-mediated transformation.
