ABSTRACT

Nanoscale materials are used to describe function of biomolecules placed in vivo or in vitro. Specifically, nanoscale-size colloids play key roles in monitoring biological functions under interfacial conditions to represent environments such as human membranes or cells. This chapter investigates the conjugation of amyloid β protein (Aβ) over gold nanocolloid in conjunction with a mechanism of fibrillogenesis, which is associated with Alzheimer's disease. The conjugation of Aβ on gold colloid was particularly interesting since the destruction of amyloid fibrils attached to gold nanoparticles was possible by exposure to weak microwave fields without harming healthy cells. For transmission electron microscopy (TEM) measurement, Formvar-coated grids were used for preparing samples of Aβ1–40-coated gold beads. The reversibility of the color change of the solution was investigated for all Aß and ovalbumin solutions between pH 4 and pH 10 by adding an adequate amount of base or acid solution as its absorption spectrum was monitored.