ABSTRACT
The chapter begins by helping to generate a collection of speckle images using extremely basic equipment to illuminate and capture data, and further to create stacks with the images and use them. It explores camera with a remote trigger will help our work, and eliminate the possibility of introducing vibrations in the setup during each touch in the camera. The chapter presents instructions of ImageJ linked together in order to create macros to implement some biospeckle analysis. It made two different approaches to develop GD algorithm: in the first, a window with a set of frames is the range where the images are subtracted and the results summed; the window after the comparisons is shifted while it fits in stack (stkSize-window). ImageJ can display, edit, analyze, process, save, and print 8-bit, 16-bit, and 32-bit images. The user should also consider the influence of other light sources during the illumination process, as the laser pointer emits some very low light.
