ABSTRACT
The ultimate objective of a genome project is the complete DNA sequence for the organism being studied. This chapter describes the techniques and research strategies that are used during the sequencing phase of a genome project, when this ultimate objective is being directly addressed. Techniques for sequencing DNA are clearly of central importance in this context, and we begin the chapter with a detailed examination of sequencing methodology. This methodology is of little value, however, unless the short sequences that result from individual sequencing experiments can be linked together in the correct order to give the master sequences of the chromosomes that make up the genome. The second part of this chapter therefore describes the strategies used to ensure that the master sequences are assembled correctly.
