ABSTRACT

A facility for plant tissue culture operations involving any type of in vitro procedures must include certain essential elements. The overall design in any laboratory organization must focus on maintaining aseptic conditions. The specific design can vary, but an effective laboratory organization for plant tissue culture must include certain elements irrespective of whether used by a scientist or group of workers. It is generally accepted that the term “plant tissue culture”, broadly refers to the in vitro cultivation of all plant parts, whether a single cell, a tissue or an organ, on nutrient medium under aseptic conditions. The basic rule essential in the planning of an efficient and functional laboratory facility is the order and continuous maintenance of cleanliness because all operations require aseptic conditions. A general guideline for setting up a facility is to focus on a design that different units and activities need to be arranged to make operational steps possible with the least amount of cross-traffic.