ABSTRACT
Chromatography has become an essential tool in biochemistry and molecular biology since the establishment of basic principles by Martin and Synge in 1941. 1 The basic principle is the same for both liquid chromatography and gas chromatography, the specific distribution of a solute between a mobile phase and a stationary phase. This distribution process may be called partitioning or adsorption, and there is an excellent collection of articles that provide basic information on chromatography. 2 The coupling of these separation technologies with mass spectrometry resulting in “hyphenated technologies” (e.g., LC-MS and GC-MS) 3,4 has been critical for progress in proteomics and metabolomics.
