ABSTRACT
Nanostructured materials are usually classified as a product with dimensions ranging from 1 nm to 100 nm. At this size, the particles may develop some unique properties and functions and may easily interact with membranes and protein complexes since the usual biological target is also at nanometric scale. In the desolvation process, a gelatin aqueous solution is added to a desolvating agent, usually an organic solvent, leading to a phase separation and a conformational change in protein structure. In the method of Emulsification-Solvent Evaporation, nanoparticles are formed by adding an aqueous solution containing both gelatin and the drug to an oil phase followed by cross-linking. Reverse-Phase Microemulsion approach uses an aqueous solution of gelatin combined with an emulsifier dissolved in a non-polar solvent. Nanoprecipitation, known as the solvent displacement or interfacial deposition, is a simple and fast technique which uses a low organic solvent amount and produces relatively small nanoparticles with a narrow polydispersity index.
