ABSTRACT
Discovered in 1989 by Stanley Fields, it utilizes the fact that the gene activation system for Saccharomyces cerevisiae yeast’s GAL4 gene is activated by a transcriptional factor possessing two distinctly separate functional domains. One of those two domains must interact with a second domain (i.e., in a genetically engineered yeast-created fusion protein containing a portion of the “known” protein) in order to cause transcription of the GAL4 gene. GAL4 is required to initiate expression of proteins that are central to galactose metabolism in that yeast. See also Two-hybrid systems, Protein, Protein interaction analysis, Gene, Gene expression, Genetic engineering, Transcription, Transcriptional activator, Coding sequence, Domain (of a protein), Fusion protein, Galactose (gal), Metabolism.
