ABSTRACT
This chapter presents advanced experiments on fluorescence. In most cases, these experiments require both steady-state and time-resolved measurements. These experiments are used to introduce readers to more advanced fluorescence measurements like polarization measurements in thin films, complex anisotropy decays, Forster resonance energy transfer (FRET), excimer and exciplex fluorescence emissions, delayed fluorescence and phosphorescence, solvatochromism, and solvent effects. Some of the experiments discussed in the chapter are: Emission Filter Selection for Fluorescence Measurements in In-Line Geometry; Raman Scattering of Different Solvents; Thermally Induced Spectral Properties; Emission Anisotropy and Chromophore Lifetime; Precautions in Fluorescence Anisotropy Measurements: Depolarization Through Reabsorption and Internal Reflections; Schematic representation of emission from the thin layer film when observed from the edge; and Photograph of F7GA in all benzene methanol used mixtures on a UV illuminator.
