ABSTRACT
In unit 2 a total of 21 experiments are included in which the Restriction Fragment Length Polymorphism (RFLP) phenomenon was first described for a mutant strain of adenovirus. Amplified Length Fragment polymorphism (AFLP) technology is a technique for fingerprinting genomic DNA. DNA fingerprinting is used to visualize DNA polymorphisms between individuals. RNA Separation of Species by Ion Exchange Column Chromatography on Methylated Albumin Kiesselguher Columns (MAK) is commonly used for fractionation of different types of RNA. Kiesselguher acts as an inert support material for albumin. The negative charges on albumin are masked by methylation, while the positive charges remain exposed and are thus available for binding nucleic acids, which are negatively charged. The Random Primer Method is 1 of the techniques employed for preparing a labelled DNA probe. In Metabolic Labelling of Proteins and Immunoprecipitation, protein synthesis can be studied normally by using labelled amino acids. In this experiment, methionine is used to trace the synthesis of a new protein. Molecular evidence for wheat rye translocations are based on identification of rye chromatic sequences in wheat by genome mapping using rye-specific repetitive sequences out of such methods as STS Marker-Based Detection of 1B/1R Translocation, STS Marker-Based Detection of Lr 26 Gene. Microsatellite Markers are also known as short tandem repeats (STRs) or simple sequence repeats (SSRs). The DNA sequences flanking SSRs are known to be conserved and have been exploited to design suitable primers for amplification of the SSR loci using PCR. The ISSR Technique is a PCR-based multi-locus marker system that employs oligonucleotide primers homologous to microsatellite or SSR sequences such as (GATA) to amplify mainly the inter-SSR regions. Construction of Genetic Linkage Maps and the Mapping of Quantitative Trait Loci (QTL) protocol gives an example of how to handle molecular data for the construction of a genetic linkage map. It is also aimed at presenting a way to prepare phenotypic data for QTL analysis and subsequent QTL mapping. This protocol represents in no case the only way to proceed, but it gives some general rules and advice when the following software is used for the analyses. Two-Dimensional Gel Electrophoresis for Analysis of Gene Expression and Metabolic Labelling for Detection of Differential Gene Expression have also described, along with special emphasis on transposons and its mutagenesis, with practical approaches.
