ABSTRACT
The simplest scheme of affinity modification may be represented as follows () P + X ⇄ (k 1 ,k − 1 ) PX → PZ(k 2 ) https://s3-euw1-ap-pe-df-pch-content-public-p.s3.eu-west-1.amazonaws.com/9781351069564/28d3cf54-07a1-4c92-84bb-5212a73c52b0/content/eq29.tif"/> where P, biopolymer; X, reagent; PX, specific complex; PZ, covalent adduct of X or of some fragments to P; and k1, k−1, and k2, rate constants of respective reactions. The scheme was considered by Baker et al. 340 and the kinetic equation corresponding to this scheme was presented by the same group of authors. 95 However, the commonly used treatment of the scheme is that proposed by Kitz and Wilson. 341 Below, we shall present this treatment performed in quasiequilibrium approximation.
