ABSTRACT
Ouchterlony gel analysis was used to evaluate the similarity and difference in the immunoprecipitin bands formed between the tumor and liver nucleolar antigens and their corresponding antibodies. Because of the need for a “preliminary” evaluation of the possible diagnostic use of the fluorescence assay for the human tumor antigens, it was important to find a series of a particular tumor type that would lend itself to a “blind” evaluation. The tumors were evaluated histopathologically by a panel of five pathologists who provided a consensus diagnosis, designation of tumor grade, and other characteristics on the basis of H&E staining. In a few benign breast tumors, nucleolar fluorescence was apparent. These nucleoli were smaller and fewer in number than in the malignant tumors. For visualization of the antigens, the gels were washed three times with isotonic saline, immersed in acetone briefly to shrink the gel, and then the gels were soaked in the antitumor nucleolar antibodies.
