ABSTRACT
This chapter devotes predominantly to the use of the isolated, perfused lung (IPL) preparation in evaluating the Phorbol Myristate Acetate (PMA) model of acute lung injury. It describes surgical techniques used to isolate lungs from this species. Isolated lungs from rabbits, dogs, and rats have been used to study the mechanism of PMA-induced lung injury. Several investigators have used the IPL to study how PMA injures the lung because this preparation affords certain advantages for this purpose. Surgical procedure tends to minimize atelectasis during perfusion. The pump to the perfusion apparatus is then turned on and the pulmonary artery and tracheal cannulae are inserted into the proper sleeves. Ventilation of lungs from animals such as rats and rabbits is commonly implemented with a small-animal respirator. To investigate the role of the neutrophil in PMA toxicity, several investigators have perfused lungs with a buffer containing a plasma expander and neutrophils.
