Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.
1. Overview 2. Host Plants in Diagnosis 3. Experimental Transmission of Viruses in Diagnosis 4. Virus Transmission through Soil and by Soil-Inhabiting Organisms in Diagnosis 5. Inclusions in Diagnosing Plant Virus Diseases 6. Virus Purification in Relation to Diagnosis 7. Serological Procedures 8. Electron Microscopy of in Vitro Preparations 9. Nucleic Acid Hybridization Procedures 10. DsRNA in Diagnosis 11. Diagnostic Methods Applicable to Viroids 12. Strategies for Implicating Virus-Like Pathogens as the Cause of Diseases of Unknown Etiology 13. Complexes of Transmission-Dependent and Helper Viruses.