ABSTRACT
Both immunohistochemical and biochemical evidence for the presence of fibronectin in articular cartilage. Clemmensen demonstrated fibronectin in human articular cartilage, and this detection also required prior treatment with hyaluronidase. Extraction with guanidinium chloride released additional fibronectin from the tissue, but proteoglycans had to be removed before detection with antibody was possible. Treatment with testicular hyaluronidase also released additional fibronectin from cartilage. Chondrocytes in cell culture do synthesize fibronectin, albeit the total amount of fibronectin produced can vary with the particular culture. Two lines of Labrador retrievers are maintained at the James A. Baker Institute at the College of Veterinary Medicine at Cornell University. They differ in susceptibility to canine hip dysplasia and the osteoarthritis which accompanies it. The collateral and sesamoid ligaments of the right knee were sectioned and 405 mm of the anterior lateral meniscus was removed from New Zealand white male rabbits.
