ABSTRACT
Stimulation of peripheral blood or splenic lymphocytes with interleukin-2 (IL-2) induces several responses, including adherence of LGL/NK cells to plastic surfaces, augmentation of NK cell cytolytic function, induction of lymphokine activated killer (LAK) cell function, and proliferation of NK cells. Vujanovic et al. developed a method for the isolation and expansion of highly purified populations of LGL/ NK cells that develop LAK activity via their selective adherence to plastic surfaces in response to rIL-2. In order to demonstrate the dependency of A-LAK cells on rIL-2 for proliferation, we examined uptake of H-TdR by A-LAK cells in response to various concentrations of rlL-2. To determine the percentage of cells in A-LAK cultures actively synthesizing DNA, we performed autoradiography on A-LAK cells pulsed with H-TdR for 2 h at different times after their induction of adherence to plastic.
