ABSTRACT
This chapter deals with the methodological approach to prepare protein- or antibody- modified liposomes by use of three types of coupling reagents: tolylene-2, 4- diisocyanate is a cross-linking reagent that can bind to two amino groups. Protein-modified liposomes can be used for various purposes. They provide models of antigenic cells or if the protein is antibody, the liposomes can be utilized for the immuno- specific delivery of drugs or other materials to the antigenic target cells. The amount of proteins in liposomes treated with various amounts of IgG in the presence or absence of l-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Homocoupling between proteins is possible but in practice polymerization of protein is minimal under these conditions. When liposomes are applied in vivo experiments, nonspecific binding with cells having Fc receptor must be taken into consideration. Agglutination of liposomes has been measured by sedimentation or by light scattering.”
