ABSTRACT
Contents 32.1 Overview ............................................................................................................................. 378 32.2 Protocols .............................................................................................................................. 379
32.2.1 DNA Extraction Procedure for RFLP Analysis Beginning with Young Leaves ................................................................................................ 379
32.2.2 Digestion of DNA with Restriction Endonucleases ............................................. 379 32.2.2.1 General Protocol for Restriction Endonuclease Cleavage .................. 379
32.2.3 Protocol for Agarose Gel Electrophoresis ............................................................ 380 32.2.4 Protocol for Southern Blotting ............................................................................. .381 32.2.5 Summary of Protocols for RFLP Probe Libraries; Transformation
with Recombinant Probes ..................................................................................... 382 32.2.6 Overnight Culture of Transformed Bacteria ......................................................... 382 32.2.7 Isolation of Bacterial Plasmids ............................................................................ .382
