ABSTRACT
Nevertheless, the hypothesis of the cytoplasmic origin of Hevea Brasiliensis latex, which would seem to have been expressed for the first time by Berthold, was partly confirmed by Milanez using optical microscopy; electron microscopy then made it possible to establish the cytoplasmic origin of latex with certainty. In the laticiferous vessels and the latex of young tissue and in the laticiferous vessels of the primary root system of Hevea, clusters of proteinic microfibrils with a double helical structure are classically displayed in lutoids by phase contrast microscopy and, above all, by electron microscopy. J. Dupont carried out the first research on the chemical composition of the lutoid membrane on lutoids separated on a discontinuous sucrose gradient using latex collected on ice. The colloidal stability of latex results from the negative charges carried by its figured elements; the most important of these are thus rubber particles and lutoids.
