ABSTRACT
Immunoelectron microscopic analysis using colloidal gold-linked second antibody demonstrated that monoclonal antibodies are able to identify the location of nuclear antigens at the ultrastructural level. Hybridoma supernatants were examined by indirect immunofluorescence using cell preparations of the human lymphoma and leukemia cell lines and peripheral blood lymphocytes. Supernatant fluids from wells with actively growing cells were screened for the presence of antibody by indirect immunofluorescence. All of the monoclonal antibodies directed against nuclear, nucleolar, and perinuclear antigens were found to be human specific and several of these antigens were lymphoid or myeloid specific. Immunocytochemical staining techniques using post-imbedding methods with colloidal gold reagents enabled the ultrastructural localization of monoclonal antibody binding in the cell nucleus. Immunoprecipitation and immunoblot procedures were performed in order to identify the molecular weight and chemical nature of the nuclear antigens recognized by specific monoclonal antibodies. A number of laboratories have used immunobiochemical techniques to study the antigenic composition of the eukaryotic nucleus.
