ABSTRACT
The use of ion-exchange chromatography for the examination of ionic or ionizable species in solution has become one of the most powerful tools available to the analyst. The use of organic solvent systems for ion-exchange separations has been investigated to only a slight extent. A system was designed in which the sample could be introduced, the gradient developed, and the column regenerated without disassembling the apparatus. The column was packed by first placing a plug of pyrex wool at the bottom of the 2.8 x 500 mm tube. The membrane in the bed support disc was removed and the disc was replaced at the base of the column. Weak organic acids will reversibly exchange on acetate form strong anion-exchange resin. Elution is carried out with acetic acid-methanol mixtures, either in a fixed concentration or a gradient mode. The acidity of phenols will be governed by the number, position, and inductivity of the substituents.
