ABSTRACT
Arylsulfatases catalyze the hydrolysis of aryl sulfates according to Reaction 1, and occur very widely distributed in the animal, plant, and microbial worlds. The three arylsulfatases were successfully separated either by electrophoresis or by ion-exchange chromatography on Amberlite® IRC-50. The enzymes were labeled I, II, and III according to the order of migration on electrophoresis. In view of accumulating evidence that tyramine was an important regulator of the synthesis of arylsulfatases in fungi and bacteria, Burns and Wynn set out to examine the effect of tyramine on the activity of the enzymes themselves. Initial studies in van Kley's laboratory which first showed the existence of arylsulfatase isoenzymes in A. oryzae also established an outline procedure for the purification of arylsulfatase II. Arylsulfatase II of A. oryzae like all other known arylsulfatases catalyzes the hydrolytic fission of the O–S bond of aryl sulfate esters to liberate the parent phenol and sulfate.
