Spectrophotometric Analysis of Some Important Groups of Drug Compounds

This chapter explains the practical aspects of the spectrophotometric analysis of drug compounds. It deals with two important groups of pyrazole derivatives. The most important pyrazolone and pyrazolidine derivatives. The tendency to enolization is the basis for the various spectrophotometric methods for the determination of derivatives in the pyrazolidine group. The furan ring itself is spectrophotometrically only slightly active. Derivative spectrophotometry has also been applied to imidazole and imidazoline derivatives. Due to the fact that chromophoric or auxochromic substituents are present in the majority of cases, spectrophotometry based on natural absorption has found wide application among the pharmaceutically-active imidazole and imidazoline derivatives. used column chromatographic separation in the determination of diazepam in a pharmaceutical formulation containing oxyphenbutazone and paracetamol. Paracetamol can easily be transformed to the o-nitrosophenol derivative, which can be measured in alkaline media at 430 nm.