ABSTRACT
In the development of the field, species or strains of fungi which were sterile or which did not usually sporulate in culture were given little consideration as fungi of potential use in large scale pure culture operations. Many of the investigators who have concerned themselves with the culture of the mycelia of edible mushrooms as well as other fleshy fungi seem to have been unduly preoccupied with the flavor and odor of the end product. Mycelia of many of the edible forms could be produced predictably in pure culture in unlimited quantities. Since large scale production of mold mycelium in submerged culture would require tremendous gallonages of water, Gray et al attempted to determine if culture medium could be prepared using sea water instead of fresh water. Growth characteristics in submerged culture were not the same for all species; some produced mycelial pellets and others produced a dispersed growth with spores.
