ABSTRACT
Recent reviews of immobilized-dye chromatography provide references to some of the approximately 500 different proteins purified using this technology. The reactive triazine dyes have been found to be particularly convenient for the preparation of immobilized-dye columns for protein purification. Such dyes include the monochloro-triazinyl dyes designated Procion H by ICI and Cibacron by Ciba-Geigy and the dichloro-triazinyl dyes designated Procion MX by ICI. These dyes cover the visible spectrum by virtue of coupling of the triazine with anthraquinone, azo, or phthalocyanine chromophores. This chapter considers the quantitation of triazine dye: protein interactions using both mobile- and immobilized- reactive blue 2. Emphasis is placed on recent experimentation, both published and unpublished, describing modulation of the affinity, accessibility, and valence of immobilized reactive blue which can be used to advantage in protein purification. It is anticipated that this discussion contains observations which pertain not only to other immobilized triazine dyes but to immobilized ligands in general.
