ABSTRACT

I1 . Derivation. Characterization. and Evaluation of Antitumor Drug and X-Ray Responses of Radiation-Retreated Human Tumor Cell Lines ........... 209 A . Derivation and Characterization of Radiation-Retreated

................................................................. Sublines 209 B . Acute X-Irradiation Responses of Parental and Radiation-

...................................................... Pretreated Sublines 210 C . Drug Responses of Parental and Radiation-Retreated Human

111 . Investigations of Mechanisms of Drug Resistance and Collateral Sensitivity in Human Tumor Sublines Derived by Fractionated

...................................................................... Acknowledgments 221

.............................................................................. References 22 l

TABLE 1 Human Tumor Cell Line Characteristics

Population doubling Colony-forming Radiation responses:

Cell line Tumor origin time (h) efnciencies D, value n value

HN-l Squamous cell carcinoma 30 + 3 13% in agaF 1 . 3 9 + 0 . 1 1 3 . 2 2 1 . 0 of the tonguez3

MCF-7 Breast carcinoma" 36 + 3 54% on plasticz8 1.47 +. 0.16 2.4 + 0.6 RT112 Transitional cell bladder 26 + 2 10% on plasticzg 1.31 + 0.07 3.6 + 0.5

carcinoma25 SuSa Testicular teratoma" 27 + 3 6% in a g d 8 0.57 + 0.02 1.4 + 0.1

HN-lIDXRll Cells Total dose

= 50G

SuSa/DXR,,Cells Total dose X-RAYS 1.5 Gy per fraction for 13 fractions =20Gy

over 15 weeks

for 10 fractions MCF-7/DXRl, Cells 8 fractions over over 30 weeks

10 weeks Total dose

RT 1 121DXR. Cells

'=ITotal dose = 48Gy

FIGURE 1 . Protocols adopted for the derivation of X-irradiation-treated human tumor cell lines in vitro.