ABSTRACT
Since Grace1 established the first insect cell line, very many cell lines have been established during the last 3 decades from various insects. In order to maintain these cell lines, various culture media have been used, because there is no single culture medium that could maintain the growth of every insect cell. Some cell lines can be maintained in relatively nutrient-poor media but others cannot. Formulation of widely usable media is one of the tasks in developing new culture media.
