ABSTRACT

Three options for oxidative pre-treatment of wastewater containing the triarlymethane dye Green S were studied and compared. Degradation of the dye was achieved using the fungal enzyme lacease, Fenton’s reagent and ozonation. All three techniques were capable of reducing absorbance at λmax by more than 98%. Biodegradability of the test solution was significantly enhanced by all three processes. The COD:CBODs ratio was reduced from 275:1 to 11:1 by Fenton’s reagent and to 2:1 by lacease and ozone. Treated solutions from all three processes showed no acute toxicity toward un-acclimatised activated sludge biomass. ADMI colour values were reduced by 81%, 97.8%, and 99.7% by lacease, Fenton’s reagent and ozonation respectively.