ABSTRACT

This volume is divided into five sections. Section I deals with preparative methodology for isolation and purification of the components of the oxy radical experimental systems use most frequently, including all three forms of SOD and several other important scavengers. Section II provides the experimenter with a choice of a dozen oxy radical generating systems which can be used for testing of scavengers or for evaluation of the effects of oxy radicals on target tissues. The reader will note that in this section, as well as in most of the rest of the volume, the orientation is primarily biochemical, biologic, and medical, rather than pure chemistry or pure physics. Nevertheless, the techniques are widely applicable to a variety of disciplines.

part |2 pages

Preparative Procedures

chapter |2 pages

Overview

chapter |6 pages

Phagocyte Nadph-Oxidase

part |2 pages

Oxygen Radical Generating Systems

chapter |4 pages

Xanthine Oxidase

chapter |10 pages

The Fenton Reaction

part |2 pages

Analytical Methods

part |2 pages

Other Radical Systems

part |2 pages

Quantitation of Superoxide Dismutase

chapter |4 pages

Cytochrome c

chapter |6 pages

Electrochemical Assay

chapter |6 pages

Adrenochrome Assay

chapter |6 pages

Pyrogallol Autoxidation

part |2 pages

Tissue Assays

part |2 pages

Cellular Phenomenon

chapter |14 pages

Oxygen Consumption

part |2 pages

Miscellaneous Methods