ABSTRACT
The total lipid content can be determined directly, with or without destruction of the sample, by measurement in various ways. Direct procedures are used in studies where only the lipid content is needed, large numbers of samples must be analyzed, and minimal equipment is available. The lipids are extracted from the milk with mixtures of fat solvents, the solvent removed, and determination is gravimetric. The reaction between vanillin and TGs at acid pH on the sulfo-phospho-vanillin reaction for the measurement of serum lipids has been applied to human milk by Dewey and Lonnerdal, Lonnerdal et al., Lemons et al., and the World Health Organization. Nakai and Le developed a turbidimetric procedure for the simultaneous determination of protein and fat in 50 µl of bovine milk. The lipids were extracted once with hexane. The method was developed by Erickson and Dunkley for the recovery of tocopherols from bovine milk.
