ABSTRACT

This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.

section 1|171 pages

Cultivating and preserving seaweeds

chapter 1|34 pages

Seaweed in high-energy environments

Protocol to move Saccharina cultivation offshore

chapter 3|18 pages

Derivation of clonal stock cultures and hybridization of kelps

A tool for strain preservation and breeding programs

chapter 4|16 pages

Cryopreservation of macroalgae

chapter 5|19 pages

Unraveling seaweeds bacteriomes

From field site to computer screen

chapter 8|19 pages

Purification of sporulation and swarming inhibitors from Ulva

Application in algal life-cycle controlling

section 2|147 pages

Chemical composition

section 3|163 pages

Cellular and molecular characterization

chapter 23|16 pages

Dynamic and microscale mapping of cell growth

Case of Ectocarpus filament cells

chapter 26|20 pages

Probing the subcellular topography of seaweeds

Transmission electron microscopy, immunocytochemistry, and correlative light microscopy