ABSTRACT
The cell biology of seaweeds is a dynamic and emerging area of research that typically requires extensive microscopy-based analyses. Transmission electron microscopy (TEM) and fluorescence microscopy represent two of the most commonly employed technologies in cell-based studies. Correlative microscopy that merges electron microscopy with light microscopy is becoming a critical tool that provides both general topological features and inclusive of high-resolution detail for cell-based research. Central to many microscopy-based imaging protocols is the proper fixation and processing of a specimen that yields optimal subcellular preservation with limited artifact production. In this chapter, a basic protocol for chemical fixation of seaweeds along with subsequent dehydration, infiltration, and embedding in plastics is described. Techniques for subsequent sectioning for TEM imaging and immunogold labeling are presented, together with modifications for applications in correlative microscopy.
Keywords: Correlative microscopy, immunolabeling, immunogold labeling, microscopy, transmission electron microscopy, seaweeds
