ABSTRACT
This chapter discusses the genomic study of culturable bacteria, which includes genomic deoxyribonucleic acid extraction of lipase-producing culturable bacteria, agarose gel electrophoresis, and quantification and purity determination of the Isolated deoxyribonucleic acid (DNA). After the isolation of DNA, quantification and the quality analysis are necessary to ascertain the approximate quantity of DNA obtained its suitability for further analysis. This is important for the various applications like digestion of DNA by restriction endonuclease enzymes or polymerase chain reaction (PCR) amplification of target DNA. Molecular genetic assessment of lipase-producing bacteria includes PCR amplification of 16S ribosomal ribonucleic acid gene and phylogenetic analysis. The chapter also discusses the effect of carbon and nitrogen sources for the growth of culturable bacteria. It describes the effect of other factors such as PH, temperature, incubation time, agitation rate, substrate, and metal Ions.
