ABSTRACT
This chapter presents specific practical approaches for the study of interacting systems. Different from the conventional interference and absorbance optical detection systems, the fluorescence detection system has many specific features that impact the data and analysis. The computational simulation of experiments with hypothesized molecular parameters, binding scheme, and affinity is a very important tool for testing an experimental design. The Job plot is a method of plotting, at constant total molar concentration, the amount of complex vs. mole fraction of B in different mixtures of A and B. The use of the entire boundary pattern as an indicator for the complex stoichiometry depends critically on the distinction between slow and fast kinetics on the time-scale of sedimentation. Lamm equation modeling is the direct fit of the recorded signal profiles across the solution column with solutions of the Lamm equation incorporating reaction terms. The non-linear regression of Lamm equation models is significantly more complex and time-consuming than the isotherms analysis.
