ABSTRACT
CONTENTS 5.1 Marine Toxins: Classification.......................................................................................... 135 5.2 Nonanalytical Detection .................................................................................................. 136
5.2.1 Mouse Bioassay..................................................................................................... 142 5.2.2 Antibody-Based Methods.................................................................................... 144 5.2.3 Radioactivity-Based Methods ............................................................................. 145 5.2.4 Cell-Based Methods.............................................................................................. 145 5.2.5 Receptor-Based Methods ..................................................................................... 145
5.3 Analytical Detection ......................................................................................................... 145 5.3.1 High-Pressure Liquid Chromatography........................................................... 146
5.3.1.1 Domoic Acid........................................................................................... 146 5.3.1.2 PSP (Paralytic Shellfish Poison, Saxitoxin,
and Analogs) .......................................................................................... 146 5.3.2 LC-MS .................................................................................................................... 147
5.4 Standards and Validation................................................................................................ 148 Acknowledgment....................................................................................................................... 149 References ................................................................................................................................... 149
If this book chapter was to describe only the methods currently available for marine toxins, it would be rather short, since even after several decades of research, the mouse bioassay is the only method for most of the known toxins. Therefore, we will comment on the philosophy of alternatives and options in the coming future.
