ABSTRACT
Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 555 20.7 General Membrane Chromatography Purification Optimization
Strategies for Viral Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 556
SHUKLA: “dk3347_c020” — 2006/6/2 — 09:38 — page 542 — #2
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 556 20.7.2 Dynamic Binding Capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 557
20.8 Downstream Processing and Plasmid DNA Capture by Anion-Exchange Membrane Chromatography . . . . . . . . . . . . . . . . . . . . . . . . 558
20.9 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 558 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 559 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 560
Gene therapy may be defined as the introduction of genetic material into cells for therapeutic purposes. The transfer of genetic material into the target cells or tissues is mediated by vectors. Such vector systems may involve viruses. These vectors are referred to as viral vectors. Alternatively, vectors may involve nonviral systems such as naked plasmid DNA. Gene therapy vectors may be administered either in vivo by direct infusion into the patient or ex vivo where the vector is introduced into target cells that are extracted from a patient. Ultimately, cells bearing a vector are infused back into the patient.
