ABSTRACT
In general, immunoassays can be defined as analytical methods in which antibodies are used for the identification and quantification of defined target analytes. At present, immunoassays are the most commonly used types of ligand-binding assays for the identification of a large variety of analytes, such as proteins, peptides, microorganisms, and low molecular weight molecules [1]. Multiplexed immunoassays (simultaneous multianalyte immunoassays), in which several analytes are measured simultaneously in a single assay, present several advantages over single-analyte assays such as reduction of analysis time, work simplification, decrease in total sample volume, and lower overall cost per test [2,3]. The ability to simultaneously measure multiple analytes in a single assay holds
enormous potential for meeting the growing demands of proteomics, diagnosis, environmental monitoring, food safety, and homeland security.
