ABSTRACT
An aliquot of the extract is injected into the GC for separation of the compounds and their subsequent analysis by a high-resolution mass spectrometer interfaced to the GC. Two exact m/z ions are monitored for each compound for their authentic identi cation. Establish the operating conditions necessary to meet the retention times (RTs) for the steroids and hormones and their labeled analogs used as internal standards. Optimize the GC conditions. e GC column and the conditions used in this method are as follow. Equivalent columns and alternate conditions may be applied if the resolution of target compounds is achieved.
