ABSTRACT
Extended depth of eld (EDF) techniques yield an image of a thick specimen at a high lateral resolution, in which the whole sample is in focus. Quite o en such an extended focus projection of a sample is all that is needed in order to answer questions of biomedical nature. For example, when tracking vesicles performing exocytosis and endocytosis within a cell, it may be su cient to follow their motion in two dimensions (2D). In an ordinary microscope, this can prove di cult when these vesicles leave the focal plane and become blurred or invisible.
