ABSTRACT
Here, NA = n sin θ i s t he numerical aperture of t he objective lens w ith aperture a ngle θ, and n is t he refractive index of the objective immersion liquid. e unwanted light that expands the apparent depth of eld is exactly what confocal imaging eliminates. us, we can view only those uorescent and lightscattering objects that lie within the depth that is given by the axial resolution of the microscope and attain the desired shallow depth of eld.
