ABSTRACT
This chapter presents the application of ion mobility-mass spectrometry (IMMS) to the separation of biomolecules as well as the elucidation of conformational
16.1 Introduction ................................................................................................ 327 16.2 The Emerging Landscape-A Historical Perspective of Ion Mobility-
Mass Spectrometry ..................................................................................... 328 16.2.1 In the Beginning Was Ion Mobility ............................................. 328 16.2.2 The Emergence of Ion Mobility-Mass Spectrometry ................. 329 16.2.3 The Biological Ion Mobility-Mass Spectrometry Era ................. 329
16.3 Ion Mobility-Mass Spectrometry Technologies as Applied to Biological Separations ................................................................................ 330 16.3.1 The Drift Tube and Traveling Wave Techniques ......................... 330 16.3.2 The Separation of Biomolecules in the IM-MS Experiment ....... 332
16.4 The Conformational Landscape of Biomolecules ...................................... 333 16.4.1 The Experimental Mobility-Mass Correlations
for Biomolecules .......................................................................... 333 16.4.2 Using Computational Methods to Assign Structural Meaning
to Empirical Data ......................................................................... 336 16.5 Navigating the Conformational Landscape-Tailoring with Chemical
Shift Strategies ........................................................................................... 338 16.6 Conclusions ................................................................................................340 Acknowledgments ..................................................................................................340 References ..............................................................................................................340
