ABSTRACT

Liquid-liquid extraction using aqueous two-phase systems (ATPSs) popularly known as aqueous two-phase extraction (ATPE) is one such method. Liquid-liquid extraction using organic-aqueous phase systems is extensively used in the chemical industry. Despite all the advantages, this method has not gained wide industrial recognition in the ™eld of biotechnology. In fact, the commonly used organic solvent systems are unsuitable for the intended purpose due to the low solubility and denaturation of biomolecules in organic solvents.