ABSTRACT
The development of quantitative real-time polymerase chain reaction (qPCR) has enabled signi- cant contributions to many aspects of molecular analysis. This advance has been contingent on instrumentation and detection chemistries, that is, signaling systems that can generate amplication-dependent uorescence, as well as instruments capable of performing the PCR cycling alongside the optical detection of emitted uorescence. Numerous congurations of the optics have been used and a similar range of detection technologies has been brought into regular use.
