ABSTRACT
Laboratories ...................................................................................... 134 6.4.2 Protocol: Monoclonal Antibody Production in CELLine
CL-1000 Flasks ................................................................................. 136 6.4.2.1 Materials ............................................................................ 138 6.4.2.2 Equipment .......................................................................... 138 6.4.2.3 Time Required ................................................................... 138 6.4.2.4 Inoculation Procedure ........................................................ 139 6.4.2.5 Cell Compartment Harvest Procedure ............................... 140
Monoclonal antibodies (mAbs) are among the most widely used tools in biomedical research, and are also of increasing value as diagnostic and therapeutic agents in clinical medicine. Production of mAbs for these diverse applications can be accomplished in vivo, usually as ascites, or in vitro, using any of a variety of systems. The choice of the production method depends upon several factors, including characteristics of the specic hybridoma, the quantity of antibody required (Table 6.1), the facilities available, local regulations concerning use of rodents for generation of ascites, cost considerations, and the specic skills or experience of the individual research team.
