ABSTRACT
Isothiocyanate Derivative of DTPA ................................... 236 9.4 Modication of Thiols and Disuldes .......................................................... 237
9.4.1 Disuldes and Thiols in IgG ............................................................. 237
9.4.2 Disulde Interchange Reaction......................................................... 237 9.4.2.1 Exemplar Protocol 8: Determination of Free Thiols
with Ellman’s Reagent (DTNB) .........................................240 9.4.3 Other Oxidation-Reduction Reactions Involving Thiols
and Disuldes ...................................................................................242 9.4.4 Alkylation of Thiols with Alkyl Halides and N-Alkyl Maleimides ... 243
9.4.4.1 Exemplar Protocol 9: Biotinylation of IgG with Maleimide-PEO2-Biotin.............................................245
