Isolation and Puri cation of DNA

Deoxyribonucleic acid (DNA) isolation and puri cation are essential techniques for molecular biology studies. The quality and integrity of the DNA isolated directly affects the results of all subsequent scienti c research.1-4 A number of methods have been developed for extraction and puri cation of DNA from different tissue types and organisms. We present several well-developed protocols here for the isolation of DNA from bacteria, lambda phages, animals, and plants and the puri cation of DNA fragments from agarose gels.