ABSTRACT
DNA Cloning is the process of moving a gene from the chromosome it occurs in naturally to an autonomously replicating vector. In the cloning process, the DNA is removed from cells, manipulations of the DNA are carried out in a test tube, and the DNA is subsequently put back into cells. Because Escherichia coli is so well characterized, it is usually the cell of choice for manipulating DNA molecules. Once the appropriate combination of vector and cloned DNA or construct has been made in E. coli, the construct can then be inserted into other cell types.
