ABSTRACT
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics,
Huazhong University of Science and Technology, Wuhan, China; Key Laboratory of Biomedical
Photonics of Ministry of Education, HuazhongUniversity of Science and Technology, Wuhan, China
Valery V. Tuchin
Saratov State University, Russian Federation; Institute of Precise Mechanics and Control of RAS,
Saratov, Russian Federation; University of Oulu, Finland
17.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 621
17.2 Mechanisms of Tissue Optical Clearing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 622
17.3 Enhancement Method of Tissue Optical Clearing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 633
17.4 Slicing Tissue with Optical Clearing Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 641
17.5 In Vivo Application of Tissue Optical Clearing Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 649
17.6 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 661
Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 662
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 663
For the last 20 years, the optical technology for molecular imaging and medical diagnosis with
high sensitivity and resolution has been a focus of life science and engineering. Noninvasive lasers
or phototherapy have been applied to many branches of medicine, including cosmetic dermatology.
However, the limited penetration of visible and near-infrared light caused by the high scattering in
turbid biological tissues affects its applicability in preclinical and clinical medicine, and other life
science [1].
