ABSTRACT

This chapter is written as if the reader is a microscope novice. Experience has taught us that it is best to assume that most students will know next to nothing about using a microscope correctly and that it is best to start from scratch. In some cases, you may have learned some bad practices that need to be corrected. This chapter also covers compound microscopes that have a field diaphragm and a condenser that can be centered and focused to achieve Koehler illumination. Many student scopes do not have these features as their condensers and field diaphragms are fixed or of limited flexibility. In the course of your career, you will encounter microscopes that have the ability to achieve Koehler illumination. At that point, this tutorial will be even more useful.